2017年12月12日 星期二
 
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【Archives of Microbiology】Hydroxy-Al and cell-surface negativity are responsible for the enhanced sensitivity of Rhodotorula taiwanensis to aluminum by increased medium pH
发布时间:2017-05-27  来源:土壤与农业可持续发展国家重点实验室  浏览:216

Zhao XQ, Bao XM, Wang C, Xiao ZY, Hu ZM, Zheng CL, Shen RF. Hydroxy-Al and cell-surface negativity are responsible for the enhanced sensitivity of Rhodotorula taiwanensis to aluminum by increased medium pH. Archives of Microbiology, 2017, doi: 10.1007/s00203-017-1387-9

Abstract

Aluminum (Al) is ubiquitous and toxic to microbes. High Al3+ concentration and low pH are two key factors responsible for Al toxicity, but our present results contradict this idea. Here, an Al-tolerant yeast strain Rhodotorula taiwanensis RS1 was incubated in glucose media containing Al with a continuous pH gradient from pH 3.1–4.2. The cells became more sensitive to Al and accumulated more Al when pH increased. Calculations using an electrostatic model Speciation Gouy Chapman Stern indicated that, the increased Al sensitivity of cells was associated with AlOH2+ and Al(OH)2+ rather than Al3+. The alcian blue (a positively charged dye) adsorption and zeta potential determination of cell surface indicated that, higher pH than 3.1 increased the negative charge and Al adsorption at the cell surface. Taken together, the enhanced sensitivity of R. taiwanensis RS1 to Al from pH 3.1–4.2 was associated with increased hydroxy-Al and cell-surface negativity.

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